Thy1-gcamp6s mice
WebbCortical imaging confirmed that type 1 expressed the cortical GCaMP6s, while type 2 did not. Conclusion: These results suggest that observation of the cross-sectioned tail in Thy1-GCaMP6s mice enabled to identify the phenotype within approximately 10 min/mouse, which reduces the cost and time for genotyping. WebbThy1-GCaMP6-WPRE transgenic mice were generated using standard techniques [23].We included the WPRE (Woodchuck hepatitis virus post-transcriptional regulatory element), which increases mRNA stability and protein expression [24,25]. Genotyping primers were 59-CATCAGTGCAGCA- GAGCTTC-39 (forward, anneals to calmodulin sequence in
Thy1-gcamp6s mice
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Webb18 okt. 2012 · The genetically encoded green fluorescent protein-based calcium sensor GCaMP provides a powerful tool for detecting calcium transients in neuronal somata, … WebbNational Center for Biotechnology Information
WebbThis study aimed to establish a new identification method for the phenotype in Thy1-GCaMP6s transgenic mice to reduce the cost and time. Methods: Tail biopsies (2 mm) … WebbThy1 Gcamp6s Male Adult Mice, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, …
Webb25 feb. 2024 · Transgenic Thy1-GCaMP6s mice were administered three doses of propofol during P7–P11 and assessed at 4 months of age. Immunostaining in the motor cortex revealed a 10–18% reduction in the density of NeuN + cells and GCaMP6 + pyramidal neurones in both L2/3 and L5 of the motor cortex after repeated propofol exposure ( Fig. … Webb13 maj 2024 · Mice were maintained in a temperature-controlled environment at Columbia University Medical Center with ad libitum access to food and water. Adult C57BL/6 mice of both sexes (8 to 12 wk old) were purchased from Jackson Laboratory. Transgenic Thy1.2-GCaMP6s mice expressing GCaMP6 slow in some DRG neurons have been previously …
Webb24 sep. 2014 · Here we present the generation and characterization of transgenic mice expressing the GECIs GCaMP6s or GCaMP6f under the Thy1 promoter. We quantified GCaMP6 expression across brain regions...
WebbCommon Name: GP4.3. These transgenic mice, made by the Genetically-Encoded Neuronal Indicator and Effector ( GENIE) Project, express the green fluorescent calcium indicator, … malla harneroWebb18 okt. 2012 · In Thy1-GCaMP2.2c mice, we observed GCaMP-expressing neurons but could not detect activated cells within an imaging window of 250 × 250 μm during a 10 … crema pantalla totalWebb24 sep. 2014 · Thy1-GCaMP6 transgenic mice for neuronal population imaging in vivo. Dana H1, Chen TW1, Hu A1, Shields BC1, Guo C1, Looger LL1, Kim DS1, Svoboda K1 … crema pan di stelle valori nutrizionaliWebbThy1 -GCaMP6-WPRE transgenic mice were generated using standard techniques [23].We included the WPRE (Woodchuck hepatitis virus post-transcriptional regulatory element), … mallah attorneyWebb4 apr. 2024 · We implanted the optical window into GP4.3 (Thy1-GCaMP6s) mice at E15.5 and observed neurotransmission among three cells, as shown in movie S5 (Fig. 2, ,I I and andJ J). The placenta is a barrier that impedes passage of chemicals such as drugs into the embryo, but the diffusion rates for molecules are difficult to assess ( 28 , 29 ). malla harnero sodimacWebb6 apr. 2024 · We had previously shown that THY1 (CD90) is a tumor suppressor in nasopharyngeal carcinoma (NPC) and that its down-regulation and loss of expression are associated with tumor metastasis, yet the mechanism leading to such effects remains unknown. In this study we show that tumor invasion could be suppressed by THY1 via … crema pantenoloWebb22 juni 2024 · These differences may be expected, as the Thy1-GCaMP6s GP4.3 mice express GCaMP6s in a subset of excitatory cells, 16 and mice expressing GCaMP6s under the CaMKIIa promoter (Ai94, tetO) have GCaMP expressed in excitatory neurons, 50 while the AAV-PHP.eB-hSyn-GCaMP6s mice express GCaMP in all neurons. 51 For this reason, … crema para batir english