Pearleygate102

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August undefined, 2024

WebAug 22, 2015 · All of the transient expression vectors used in our experiments, including pDONR221, pEarleyGate101, pEarleyGate102, pEarleyGate201, pYFPN, and pYFPC, were kindly provided by Professor Wang from the Southern Canada Agriculture Plant Protection and Food Research Center, and the vectors were not modified. WebJun 15, 2024 · The full-length OSGLIP1 cDNA was cloned into pEarleyGate102 ( 35Spro: OSGLIP1-CFP ). For tobacco transient transformation, the constructs in strain GV3101 were coinfiltrated into the Nicotiana benthamian epidermis, and the endoplasmic reticulum (ER) marker OFP-HDEL was used for co-localization. Fluorescence was determined 3 days …

Table of Vectors - IU

WebFor co-IP assays and transient expression assay, RH12 gene fragment was inserted into pCAMBIA1300-CFP by using the ClonExpress II One Step Cloning Kit (Vazyme, C112) to … WebJun 23, 2024 · Recently, we have cloned a histidine-rich calcium-binding protein gene (TaHRC) as the causal gene for Fhb1 and demonstrated that the wild-type allele of TaHRC conditions FHB susceptibility and a... tesmat kit

pEarleyGate 101 - IIS7

WebHowever, their roles in Potyvirus cell-to-cell movement are not known. The plasma membrane (PM)-associated Ca2+binding protein 1 (PCaP1) interacts with the P3N- PIPO … WebJul 7, 2024 · 3 beds, 2 baths, 2044 sq. ft. house located at 2102 Gate Pointe Way, Arlington, TX 76018 sold on Jul 7, 2024 after being listed at $215,000. MLS# 13593692. You won’t … WebVisit ChemicalBook To find more pEarleyGate 101() information like chemical properties,Structure,melting point,boiling point,density,molecular formula,molecular weight, physical properties,toxicity information,customs codes. You can also browse global suppliers,vendor,prices,Price,manufacturers of pEarleyGate 101(). At last,pEarleyGate … rodriguez samon

pEarleyGate 102 Sequence and Map - SnapGene

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WebTair Accession. Vector:1007964469. Description. pEarleyGate 201 allows for rapid recombinational cloning of cDNAs from Gateway entry vectors to be epitope tagged with … rodriguez ufc mujerWeband pEarleyGate102, respectively, for BiFC and cellular localization studies, creating pYFPN-p5, pYFPN-p3, pYFPC-p5, pYFPC-p3, YFP-p3 and CFP-p5. A construct expressing SV40NLS-mCherry was used in cotransfection experiments to label the nucleus [32]. In the Co-IP assay, the full-length ORFs of p5 and p3 were inserted into the rodriguez ruiz javier gonzalo

"WebA plant RNA virus activates selective autophagy in a UPR-dependent manner to promote virus infection Fangfang Li1,2* , Changwei Zhang1,3* , Ziwei Tang1,4, Lingrui Zhang1,4, Zhaoji Dai1,4, Shanwu Lyu1,3, Yinzi Li1, Xilin Hou3, Mark Bernards4 and Aiming Wang1 1London Research and Development Centre, Agriculture and Agri-Food Canada, London, … " - Pearleygate102

Pearleygate102

WebCFP-REM1.2 obtained by fusion PCR of pEASY-REM1.2 and pEarleyGate102 was cloned into pEarleyGate100 to yield CFP-REM1.2 construct. All plasmids generated in this study were veriﬁed by sequencing. New Phytologist2024 The Authors New Phytologist 2024 New Phytologist Trust (2024) 225: 2122–2139 www.newphytologist.com New … WebMar 26, 2009 · The coding regions of AtBI-1 or AtFAH2 were cloned into the pEarleyGate102 vector to construct AtBI-1-CFP or AtFAH2-CFP, respectively (Earley et al., 2006). The coding region of AtCb5-B was also cloned into pEarleyGate104 to construct YFP-AtCb5-B. The CFP excitation is at 442 nm.

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WebpEarleyGate102 Inquiry Product Name pEarleyGate102 Cat# PEV-89 Description An Agrobacterium dual expression plasmid. Vector Type Gateway Vector, Plant Expression … WebMay 16, 2013 · The 6K2 cistron of TuMV was amplified by PCR. Gateway technology with the entry vector pDONR201 and destination vectors pEarleygate101 and pEarleygate102 was again used to produce plasmids 6K2-HA-YFP and 6K2-CFP, respectively. The plasmids including mTalin-CFP containing the mouse talin (mTalin) sequence , was described …

WebMar 31, 2024 · Zestimate® Home Value: $24,100. 10102 Parkgate Ave, Cleveland, OH is a multi family home that contains 2,773 sq ft and was built in 1911. It contains 2 bedrooms … WebJun 17, 2024 · In colocalization assay, the full-length ORF of p2 was cloned into pEarleyGate102 (CFP) and that of Atcoilin, Atcoilin1, Atcoilin2, and Atcoilin3 into …

WebIf you use pEarleyGate vectors in work you intend to publish, please cite: Earley, Keith, Jeremy R. Haag, Olga Pontes, Kristen Opper, Tom Juehne, Keming Song, and Craig S. … WebOct 17, 2024 · Insertions in the resulting pDONR clones were verified by DNA sequencing, and then the linearized intermediate pDONR221 plasmids carrying inserts were further transferred into Gateway-compatible vectors: pGADT7-DEST, pGBKT7-DEST, pEarleygate101, pEarleygate102, pEarleygate103, pEarleygate104, 35S-YN gateway, 35S-YC gateway, …

WebZestimate® Home Value: $359,400. 2102 Gate Pointe Way, Arlington, TX is a single family home that contains 2,044 sq ft and was built in 1999. It contains 3 bedrooms and 2 …

WebNov 15, 2024 · To create the 35S pro:VCC:CFP transgene, a fragment of the At2g32280 cDNA that spanned the coding region (TAIR10 At2g32280 cDNA coordinates 170 to 664) was cloned into the pEarleyGate102 vector (Earley et al., 2006). rodriguez sandra i mdWebSimilarly, full-length ABI5, deletion mutants, and point mutations of ABI5 were cloned into the pEarleyGate102 vector to generate the C-terminal-tagged cyan fluorescent protein (CFP) and HA fusions (ABI5-CFP). For simplification, the HA tag was not included in the names of the fusion proteins throughout the text. tesmart usb-c kvmWebE5fSpeI and E5r2, and fragment CFP was amplified from plasmid pEarleyGate102 (ABRC) using primers E5CFPf3 and CFPr4SpeI. These two PCR products were combined in a … rodriguez urology grand rapidsWebPEARLEYGATE102 : plasmid : pEarleyGate 102 allows for rapid recombinational cloning of cDNAs from Gateway entry vectors to produce C-terminal fusions of encoded proteins in-frame with CFP HA. This vector is useful for cell localization studies and uses an enhanced CaMV 35S promoter to drive expression. More information concerning pEarleyGate ... tesmec middle eastWeb13102 Peppergate Ln, Houston, TX 77044 is currently not for sale. The 1,645 Square Feet townhouse home is a 3 beds, 3 baths property. This home was built in 2006 and last sold … tesmat teslaWebName. pEarleyGate102. Type. plasmid. Tair Accession. Vector:1007964466. Description. pEarleyGate 102 allows for rapid recombinational cloning of cDNAs from Gateway entry … tesmianWebJul 26, 2016 · For transient expression analysis in plant cells, the full-length coding sequence of Arabidopsis AtRH9 was transferred by recombination into the binary destination vectors pEarleyGate101 56 to generate plant expression vector for transient expression of AtRH9-YFP (yellow fluorescence protein) or pEarleyGate102 to produce AtRH9-CFP (cyan ... rodriguez zambrano manta