Web23 jun. 2006 · Particle size distribution was determined by two independent methods, coulter counting and SEM pictures, with fair agreement. Binding strength data of cation-exchange resins as a function of ionic strength depends on the protein, but binding and elution at high salt concentration may in general be performed with MacroPrep resins. Web1 nov. 2016 · The recombinant protein has theoretical pI 4.87 (fusion protein = 4.67 and target protein = 6.18). I used both anion exchange (Hitrap Q HP and DEAE) and cation exchange column (Hitrap SP HP and CM ...
A case study of the mechanism of unfolding and aggregation of a ...
Web2.1.4 Ion Exchange Systems. Ion exchange resins consists of two main types, i.e., cation exchange resins, that exchange positively charged ions, such as sodium, for calcium, and anion exchange resins, that exchange negatively charged ions, such as chloride, for arsenic. Resins are usually made from the polystyrene polymer backbone and differ ... Web15 okt. 2024 · Ion-exchange chromatography is an incredibly versatile method for protein purification, which is critical to certain experiments such as surface plasmon … sign in pathfinder international
Cation Exchange Resin - an overview ScienceDirect Topics
Web15 apr. 2024 · Ion intensities are on the top right corners. Aqueous CEX eluent at 0.3 ml/min electrosprayed directly (top panel) and post-column T-mixed with the organic-containing, acidic solvent at 0.1 ml/min (middle to bottom panels). Top-down analysis was performed by increasing in-source voltage and fragmenting all entering multiply charged protein ions. Web28 aug. 2015 · As a result, proteins immobilized on an ion exchange column can be eluted either by increasing the s alt concentration or by altering the pH of the binding buffer, or a combination of the two. This lab activity involves using a protein mix, consisting of hemoglobin and Cytochrome C, and running ion- exchange chromatography to separate … Web14 apr. 2024 · Protein-Pak HR Ion-Exchange Glass Columns The naturally hydrophilic polymer reduces non-specific adsorption, resulting in quantitative recovery of proteins … sign in payentry