Cisplatin assay

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August undefined, 2024

WebMay 26, 2024 · Cisplatin is a well-known chemotherapeutic drug for solid tumors and leukemia with muscle toxicity, and long-term cisplatin medication can induce aberrant ROS accumulation in muscle cells and result in systemic muscle impairments ( Sakai et al., 2014; Conte et al., 2024 ). WebSep 18, 2024 · Cisplatin is a medication used to manage and treat solid tumors and hematologic malignancies. It is in the alkylating agent class of cytotoxic medications. This activity reviews the indications, action, and …

Does anyone know the best assay to prepare cisplatin

WebSep 30, 2024 · Cisplatin (cis-diamminedichloridoplatinum (II), CDDP) is one of the most commonly used antineoplastic drugs worldwide. The platinum-based compound has been in clinical use for more than 40 years and is a cost-effective first-in-line treatment against several solid cancers including ovarian, head and neck and testicular cancer ( 1 ). WebAdjuvant cisplatin, 70 mg every three weeks, was given, for a total of 1680 mg. In February 1979, while he was receiving cisplatin, fever developed, accompanied by dark urine, a … phoenix to hilton head flights

Cisplatin Injection: MedlinePlus Drug Information

WebOct 25, 2016 · The MTT assay is currently the most extensively used method for IC50 measurements. Here, we dissected the critical reasons behind MTT-dependent IC50 … WebCisplatin is a platinum-based agent whose nephrotoxicity is thought related to the chloride in the cis position. Cisplatin gains entry into tubular cells via uptake by the OCT-2 … WebOct 22, 2024 · Cisplatin is a commonly used anticancer drug, but nephrotoxicity is a dose-limiting adverse effect. Recent experimental and clinical observations have demonstrated that multiple injections of... phoenix to incheon

Mechanism of Cisplatin-Induced Cytotoxicity Is Correlated to

Development and validation of a LC-MS/MS assay for ... - PubMed

WebApr 11, 2024 · Cisplatin is a widely used chemotherapeutic agent for treatment of various cancers. However, treatment with cisplatin is associated with drug resistance and … WebBased on my testing, the best way is to dissolve cisplatin in 0.9% NaCl, and this approach can keep cisplatin stable not only at room temperature but also at 37-41°C. The solubility of... how do you get folliculitisWebNov 1, 2008 · Cisplatin is an anti-tumor agent widely employed in cancer chemotherapy. A specific and selective method for the quantitative determination of cisplatin in human plasma and its applications to ... how do you get fluid out of your legs

"WebMar 15, 2024 · Here, MGC-803 and SGC-7901 gastric cancer cells were treated with different concentrations of cisplatin. Comet assay was used to detect DNA damage and the results confirmed the dose-effect of cisplatin-induced DNA damage in gastric cancer cells. URI knockdown cell lines were established with siRNA transfection. Cell viability and … " - Cisplatin assay

Cisplatin assay

WebSep 15, 2024 · Cisplatin Injection (cisplatin injection (cisplatin (cisplatin injection) injection) ) is a sterile aqueous solution, available in 50, 100 and 200 mL multiple dose … WebMar 1, 2024 · The developed and validated assay presented here is a highly sensitive LC-MS/MS method developed and validated for the quantitative determination of cisplatin following derivatization with diethyldithiocarbamate (DDTC) in order to detect platinum (Pt) of cisplatin, suitable for pharmacokinetic studies in rats and to further use it to study …

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WebCisplatin is a key chemotherapeutic agent for the treatment of esophageal cancer. We examined the intracellular localization of cisplatin in esophageal cancer cell lines and determined their sensitivity to cisplatin using in-air micro-PIXE (particle induced X … WebAug 6, 2015 · The chemotherapeutic use of cisplatin is limited by its severe side effects. In this study, by conducting different omics data analyses, we demonstrated that cisplatin …

WebOct 25, 2016 · The MTT assay is currently the most extensively used method for IC50 measurements. Here, we dissected the critical reasons behind MTT-dependent IC50 inconsistencies. We showed that IC50 errors caused by the technical deficiencies of the MTT assay are large and not adjustable (range: 300-11,000%). WebAbstract Cisplatin (DDP) is a common therapeutic option for non-small cell lung carcinoma (NSCLC). However, some patients fail to respond to the DDP chemotherapy. ... Cells were lysed with radioimmunoprecipitation assay (RIPA) buffer and incubated with anti-USP14 (ab70311; Abcam), anti-EGFR (ab52894; Abcam), or normal IgG (sc-2027; Santa Cruz ...

WebNov 29, 2024 · Cisplatin and pemetrexed response were quantified by proliferation and colony formation assay. Results Pemetrexed treatment of parental A549 cells increased mitochondrial mass over time. FACS-sorted paraclonal Mito-High cells featured increased mitochondrial mass and mitochondrial DNA copy number compared to the Mito-Low cells. http://www.pharmacopeia.cn/v29240/usp29nf24s0_m17910.html

WebIntroduction: Cisplatin has been indicated for several malignancies all over the world for many years. Increasing patient tolerance for high dose of chemotherapeutics and …

WebUsing transporter-mediated cellular uptake assay, we identified wedelolactone (WEL), a medicinal plant-derived natural compound, is a competitive inhibitor of OCT2 and a noncompetitive inhibitor of MATE1. Wedelolactone showed a selectivity to inhibit OCT2 rather than MATE1. how do you get fonts on computerWebElectron microscopywas used to observe ultrastructure changes in cell. Results: The depletion of reduced glutathione caused by cisplatin and the inactivation of glutathione peroxidase played the vital role in the underlying mechanism. how do you get follower emotes on twitchWeb(A,B) MTT assay. The cisplatin-resistant cells and their parental cells were exposed to different concentrations of cisplatin for 72 h and viability was related to untreated … phoenix to joshua tree caWebCisplatin An anticancer agent WARNING: This product can expose you to chemicals including Cisplatin, which is [are] known to the State of California to cause cancer. For … how do you get food coloring out of clothesAfter observing the enhanced cell-killing efficacy of the combination of CDDP and BV10 in various cancer cell lines and studying cell death processes, we further investigated the anti-cancer effect of this combination in the xenograft mouse models of lung, ovarian and cervical cancers. The typical dose of … See more The results of the effect of the combination of CDDP with BV10 on in vitro cell viability and clonogenic death are shown in Fig. 3. Figure 3A shows the … See more To demonstrate the DET mechanism of the combination, we made real-time measurements of the reaction between CDDP and BV10 … See more Figure 4A shows representative images of HeLa cells treated by CDDP with/without the combination with BV10 for 12 h, where apoptotic cells were … See more how do you get food stamps in oregonWebMar 1, 2024 · Cisplatin (cis-diamminedicholroplatinum II, Fig. 1) is a platinum complex bound to two chloride atoms and two ammonia molecules which makes it highly soluble … how do you get followers on onlyfansWebSep 23, 2016 · The subsequent intracellular signaling pathway analysis indicated that pAkt and p62 expression levels correlated with alterations in the IC50 values for cisplatin in ovarian cancer, providing an... how do you get foot ulcers